三氧化二砷对胃癌NCI-N87细胞增殖的影响Effect of Arsenic trioxide on Proliferation of gastric cancer cells NCI-N87
郭皓;梁军;张际绯;
摘要(Abstract)：
目的研究三氧化二砷(arsenic trioxide,As2O3)对胃癌细胞系NCI-N87的细胞增殖与凋亡的影响。方法用50umol/L的As2O3处理NCI-N87细胞,培养24h后,用MTT法检测As2O3对NCI-N87细胞的增殖影响,和TUNEL法检测细胞经过As2O3处理后细胞凋亡情况。结果 50umol/L的As2O3作用24h后NCI-N87细胞的细胞增殖率是21.3%。经过As2O3处理的NCI-N87细胞相比没有进行加药处理的细胞出现了大量的细胞凋亡。As2O3作用24h后NCI-N87细胞的不同视野细胞凋亡率分别是37.8%、34.00%、35.40%、36.8%(P<0.01),正常没有加药培养的NCI-N87细胞的细胞凋亡率是6.20%、5.00%、7.60%、7.00%(P<0.01)。结论通过As2O3诱导其细胞凋亡,使胃癌NCI-N87细胞的细胞增殖有明显的抑制作用。
关键词(KeyWords)： 三氧化二砷;胃癌NCI-N87细胞;凋亡
基金项目(Foundation): 牡丹江师范学院2014年研究生学术科技创新项目(yjsxscx2014-08mdjnu)
作者(Author): 郭皓;梁军;张际绯;
Email:
DOI: 10.13799/j.cnki.mdjyxyxb.2016.02.002
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