Nucleostemin特异性RNA干扰对肿瘤细胞增殖能力的影响NUCLEOSTEMIN SPECIFIC RNA IN SEVERAL TUMOR CELLS INFLUENCED THE CELLS PROLIFERATION
蔡子微,刘思金,孙丽秋,劳为德
摘要(Abstract)：
目的 :检测肿瘤细胞Nucleostemin(NS)的表达 ,研究NS特异性RNA干扰对Hela细胞体内外增殖的影响。方法 :提取 6种肿瘤细胞总RNA ,用RT -PCR和Northernblot方法检测NS的表达。用NS特异性siRNA表达载体转染Hela细胞 ,观察转染的Hela细胞 (简称NS -SiRNA -Hela细胞 )体内外增殖的变化。结果 :6种肿瘤细胞中NS显高表达。体外培养的NS -siRNA -Hela细胞中NS表达显著降低 ,G0 /G1期细胞百分率显著升高。体内致瘤实验显示 ,NS -siRNA -Hela细胞在裸鼠体内增殖显著降低。结论 :NS在肿瘤细胞中高表达具有普遍性。NS特异性RNA干扰使Hela细胞进入S期受阻 ,并可明显降低Hela细胞体内外的增殖能力
关键词(KeyWords)： Nucleostemin;干细胞;肿瘤细胞;RNA干扰;细胞周期
基金项目(Foundation):
作者(Author): 蔡子微,刘思金,孙丽秋,劳为德
Email:
DOI: 10.13799/j.cnki.mdjyxyxb.2004.03.001
参考文献(References)：
- 1 TsaiRY ,McKayRD .Anucleolarmechanismcontrollingcellprolif erationinstemcellsandcancercells[J].Genes&Dev,2002,16(23):2991～3003
- 2 蔡子微,刘思金,胡国法,等.NS特异性siRNA真核表达载体的构建[J].牡丹江医学院学报,2003,24(3):1～4
- 3 刘思金,薛延,劳为德,等.人类骨桥蛋白(hOPN)在细胞增殖中的功能研究[J].高技术通讯,2003,13(2):25～28
- 4 Liusijin,Xueyan,Laoweide,etal.Theeffectofhumanosteopon tinontheproliferation,transmigrationandexpressionofMatrixMetallproteinase-2and9ofOsteosarcomaCellsinIntro[J].Chi neseMedicalJournal,2004,inpress
- 5 PoolmanRA ,Gilchrist,BrooksG .Cellcycleprofilesandexpressionofp21cIPIandp27KIP’duringmyocytedevelopment[J].Interna tionalJournalofCardiology,1998,67:133～142
- 6 SunY ,KimH ,ParkerM ,etal.LackofsuppressionoftumorcellphenotypebyoverexpressionofTIMP -3inmouseJB6tumorcellsidentificationofatransfectantwithincreasedtumorigenicityandin vasiveness[J].AnticancerRes,1996,16(1):1～7
- 7 TakagiS ,McfaddenML ,HumphreysRE ,etal.Detectionof5-bromo-2-deoxyuridine(BruUrd)incorporationwithmonoclonalanti-BruUrdantibodyafterdeoxyribonucleasetreatment[J].Cy tometry,1993,14:640～648
- 8 BaddooM ,HillK ,WilkinsonR ,etal.Characterizationofmes enchymalstemcellsisolatedfrommurinebonemarrowbynegativeselection[J].JCellBiochem,2003,89(6):1235～1249
- 9 BernatchezR ,BelkacemiL ,RassartE ,etal.Differentialexpres sionofmembraneandsolubleadenylylcyclaseisoformsincytotro phoblastcellsandsyncytiotrophoblastsofhumanplacenta[J].Pla centa,2003,24(6):648～657
- 10 Chambers1,ColbyD ,RobertsonM ,etal.FunctionalexpressioncloningofNanog,apluripotencysustainingfactorinembryonicstemcells[J].Cell,2003,113(5):643～655
- 11 SuiG ,SoohooC ,AffarEB ,etal.ADNAvector-basedRNAitechnologytosuppressgeneexpression.inmammaliancells[J].Proc.Acad.Sci.USA ,2002,99:5515～5520
- 12 YuJY ,DeRuiterSLandTurnerDL .RNAinterferencebyexpressionofshortinterferingRNAsandhairpininmammaliancells[J].Proc.Acad:Sci.USA ,2002,99:6047～6052
- 13 PaulCP ,GoodPD ,Winer1,etal.EffectiveexpressionofsmallinterferingRNAinhumancells[J].NatureBiltechnol,2002,20:505～508
- 14 BernardiR ,PandolfiPP .Thenucleolus:atthestemofimmortali ty[J].NatMed,2003,9(1):24～25