张腊梅;李竹;李子涛;袁琳;刘建廷;李彩娟;

• 1:牡丹江医学院
• 2:牡丹江医学院附属红旗医院

摘要(Abstract)：

目的本文探讨环磷酰胺Cyclophosphamide对乳腺癌增殖、迁移和凋亡的影响及其机制研究。方法运用基因表达谱交互式分析(GEPIA)数据库分析乳腺癌组织中的基质金属蛋白酶9(mtrix metalloproteinases 9,MMP9)基因差异表达及预后情况。MTT法检测人乳腺癌细胞MCF-7细胞的增殖活性,TUNEL法检测细胞凋亡情况,划痕愈合实验检测MCF-7细胞水平迁移能力;反转录-聚合酶链反应PCR(RT-PCR)检测MMP9基因表达情况。结果 MMP9在乳腺癌中高表达,其基因表达量与乳腺癌患者总体生存率有关。CTX抑制乳腺癌细胞MCF-7的增殖、迁移,促进细胞凋亡,下调MMP9表达。结论 CTX能有效抑制乳腺癌MCF-7细胞增殖和迁移,诱导细胞凋亡,其机制可能与调控MMP9表达有关。

关键词(KeyWords)： 乳腺癌;细胞增殖;迁移;凋亡;环磷酰胺

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省科学基金项目(H2017078);; 黑龙江省省属高等学校基本科研业务费科研项目(2017KYYWFMY0653;2017KYYWFMY0684;2018KYYWFMY0040)

作者(Author): 张腊梅;李竹;李子涛;袁琳;刘建廷;李彩娟;

Email:

DOI: 10.13799/j.cnki.mdjyxyxb.2020.01.001

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