舒芬太尼对人肺腺癌细胞A549细胞增殖和凋亡的影响Effect of sufentanil on proliferation and apoptosis of human lung adenocarcinoma A549 cells
袁益民;芮燕;金孝岠;
摘要(Abstract)：
目的观察不同浓度梯度的舒芬太尼体外处理人肺腺癌细胞A549后对细胞增殖和凋亡的影响。方法采用CCK-8法检测舒芬太尼(0.01、0.02、0.04μg/m L)处理A549细胞48 h后的增殖情况,采用Annexin V-FITC/PI双染法检测舒芬太尼处理A549细胞48 h后的细胞凋亡情况,Western blotting检测各浓度舒芬太尼作用A549细胞48h后的凋亡相关蛋白p53、Bcl-2和Bax的表达水平。结果舒芬太尼能够抑制A549细胞增殖以及诱导其凋亡,且增值率和凋亡率的变化呈剂量依赖性(P<0.05)。与对照组相比,各浓度舒芬太尼组A549细胞的凋亡率均有不同程度升高(P<0.05),且p53和Bax蛋白水平表达升高,而Bcl-2蛋白表达量降低(P<0.05)。结论舒芬太尼可抑制人肺腺癌A549细胞增殖并促进其凋亡,可能与舒芬太尼能够上调p53和Bax蛋白的表达以及下调Bcl-2蛋白的表达有关。
关键词(KeyWords)： 舒芬太尼;肺癌;增殖;凋亡
基金项目(Foundation):
作者(Author): 袁益民;芮燕;金孝岠;
Email:
DOI: 10.13799/j.cnki.mdjyxyxb.2018.03.002
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