转化生长因子对大鼠内侧副韧带损伤愈合的影响EFFECT OF TRANSTORMING GROWTH FACTOR BETA 1 ON THE HEALING RAT MEDIAL COLLATERAL LIGAMENT
宋连杰;姜大鹏;吴红;李昭铸;
摘要(Abstract)：
目的:观察转化生长因子(TGF-β1)对大鼠内侧副韧带(MCL)损伤愈合的影响。方法:成年雄性SD大鼠40只(体重:345～400g),随机分实验组(A)与对照组(B),每组20只。制备右膝关节内侧副韧带(medical collateral ligament,MCL)断裂模型,实验组继续给予生长因子,对照组给予盐水,至术后第4周,将愈合处韧带取下,进行愈合处肌腱组织学检查及生物力学检查。结果:电镜下见转化生长因子组胶原纤维排列整齐,而对照组胶原纤维排列紊乱。愈合的MCL组织结构比对照组好,生物力学性能也更好。转化生长因子组愈合的MCL生物力学检测级载负荷和刚度均大于对照组。结论:转化生长因子对韧带的愈合有促进作用。因此,本实验为韧带损伤实验研究及临床治疗韧带损伤提供理论依据。
关键词(KeyWords)： 转化生长因子;内侧副韧带;愈合;大鼠
基金项目(Foundation):
作者(Authors): 宋连杰;姜大鹏;吴红;李昭铸;
DOI: 10.13799/j.cnki.mdjyxyxb.2007.02.009
参考文献(References)：
- 1Sheng JG,BF.Z.Expression of transforming growth factor-?in healing process of insheathed injury to chicken flexor digitorum ten-don.Chin J Orthop Trauma.2005,7:256-258.
- 2Sha DF,Xin CT,Yang XX ea.Experimental study on basic fibro-blast growth factor combined slow-releasing degradable membrane to prevent tendon adhesion.Chinese J Reparative and Reconstructive Surgery.2004,18:148-151.
- 3Batten ML,Hansen JC,LE.D.Influence of dosage and timing of application of platelet-derived growth factor on early healing of the rat medial collateral ligament.J Orthop Res.1996,14:736-41.
- 4Watanabe N,Woo SL,Papageorgiou C ea.Fate of donor bone mar-row cells in medial collateral ligament after simulated autologous transplantation.Microsc Res Tech.2002,58:39-44.
- 5Zhou JS,Zhang CC,Pan GP ea.An experiment on transplantation of cryopreserved bone-anterior cruciate ligament(ACL)-bone al-lograft.Chin J Orthop Trauma.2004,6:546-549.