EB病毒感染套细胞淋巴瘤细胞株的表型研究PHENOTYPE STUDY OF EPSTEIN-BARR VIRUS INFECT MANTLE CELL LYMPHOMA CELL LINES
金在顺;马璨粲;徐晓焱;寺本典弘;李志强;安锦丹;曹永;吉野正;
摘要(Abstract)：
目的:建立EBV潜伏感染的体外理想模型。方法:我们利用新霉素耐性的重组遗传基因EBV(NeoR EBV)对套细胞的细胞株SP53进行感染,用G418筛选,选出100%EBV阳性的MCL细胞株SP53/EBV。结果:此细胞株表达EBER1、EB-NAs、LMP1,表现出LCL型的EBV潜伏感染的免疫表型.结论:这次我们建立的SP53/EBV细胞株,具有低增殖功能,没有诱导出活性指标等特点,可考虑为机体内EBV休眠期B细胞具有的特征,期待此细胞株可成为研究体内EBV潜伏感染的良好模型。
关键词(KeyWords)： 套细胞淋巴瘤;EB病毒;潜伏感染
基金项目(Foundation): 黑龙江省科技厅留学归国人员攻关计划项目(No.LC06C25);; 黑龙江省教育厅海外学人重点科研资助项目(No.1152hz36);;
作者(Author): 金在顺;马璨粲;徐晓焱;寺本典弘;李志强;安锦丹;曹永;吉野正;
Email:
DOI: 10.13799/j.cnki.mdjyxyxb.2007.06.046
参考文献(References)：
- 1 Harris NL,Jaffe ES,Stein H,Banks PM,Chan JK,Cleary MLet al:A revised European-American classification of lymphoid neoplasms:aproposal fromthe International Lymphoma Study Group.Blood 5:1361-92,1994.
- 2 Amin HM,McDonnell TJ,Medeiros LJ,et al:Characterization of 4mantle cell lymphoma cell lines.Arch Pathol Lab Med 4:424-31,2003.
- 3 Jin Z,Teramoto N,Hayashi K,et al:.:CD40 Ligand stimulation in-hibits the proliferation of mantle cell lymphoma lines.Anticancer Res.2004 Mar-Apr,24(2B):691-7.
- 4 Kieff E:Epstein-Barr virus and its replication;In Fields Virology,Fields BN,Knipe DMand Howley PMeds,Lippincott-Raven,Phila-delphia,PA,:pp2343-2396,1996.
- 5 Hayashi K,Jin Z,Onoda S,et al::Rabbit model for human EBV-as-sociated hemophagocytic syndrome(HPS):Sequential autopsy analysisand characterization of IL-2 dependent cell lines established fromher-pesvirus papio-induced fatal rabbit lymphoproliferative diseases withHPS.Am J Pathol.May;162(5):1721-36,2003.
- 6 Koirala TR,Hayashi K,Jin Z,et al.:Induction and prevention of vi-rus-associated malignant lymphoma by serial transmission of EBV-related virus from cynomolgus by blood transfusion in rabbits.ActaMed Okayama.2004 Apr;58(2):67-74.
- 7 Thorley-Lawson DA,Miyashita EMand Khan G:Epstein-Barr virusand the B cell:thats'all it takes.Trends Microbiol(1996)4,204-208.
- 8 Miyashita EM,Yang B,Babcock GJ et al:Identification of the site ofEpstein-Barr virus persistence in vivo as a resting B cell.J Virol(1997)71,4882-4891.
- 9 Babcock GJ,Decker LL,Freeman RB,et al:Epstein-Barr virus-infected resting memory B cells,not proliferating lymphoblasts,accu-mulate in the peripheral blood of immunosuppressed patients.J ExpMed(1999)190,567-576.
- 10 Tomita Y,Avila-Carino J,Yamamoto K,et al:Recognition of B-CLL cells experimentally infected with EBV by autologous Tlympho-cytes.Immunol Lett(1998)60,73-79.
- 11 Teramoto N,Gogolgy P,Nagy N,et al:Epstein-Barr virus infectedB-CLL cells express the virally encoded nuclear proteins but they donot enter the cell cycle.J Hum Virol(in press).
- 12 Daibata M,Kubonishi I,Eguchi T,et al:The establishment of Ep-stein-Barr virus nuclear antigen-positive(SP-50B)and Epstein-Barr virus nuclear antigen-negative(SP-53)cell lines with t(11;14)(q13;q32)chromosome abnormality from an intermediatelymphocytic lymphoma.Cancer(1989)64,1248-1253.