EB病毒感染套细胞淋巴瘤细胞株的表型研究PHENOTYPE STUDY OF EPSTEIN-BARR VIRUS INFECT MANTLE CELL LYMPHOMA CELL LINES
金在顺;马璨粲;徐晓焱;寺本典弘;李志强;安锦丹;曹永;吉野正;
摘要(Abstract)：
目的:建立EBV潜伏感染的体外理想模型。方法:我们利用新霉素耐性的重组遗传基因EBV(NeoR EBV)对套细胞的细胞株SP53进行感染,用G418筛选,选出100%EBV阳性的MCL细胞株SP53/EBV。结果:此细胞株表达EBER1、EB-NAs、LMP1,表现出LCL型的EBV潜伏感染的免疫表型.结论:这次我们建立的SP53/EBV细胞株,具有低增殖功能,没有诱导出活性指标等特点,可考虑为机体内EBV休眠期B细胞具有的特征,期待此细胞株可成为研究体内EBV潜伏感染的良好模型。
关键词(KeyWords)： 套细胞淋巴瘤;EB病毒;潜伏感染
基金项目(Foundation): 黑龙江省科技厅留学归国人员攻关计划项目(No.LC06C25);; 黑龙江省教育厅海外学人重点科研资助项目(No.1152hz36);;
作者(Authors): 金在顺;马璨粲;徐晓焱;寺本典弘;李志强;安锦丹;曹永;吉野正;
DOI: 10.13799/j.cnki.mdjyxyxb.2007.06.046
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