王闯;王猛;聂影;文怡欣;刘野;鄂志野;鞠宝玲;张红军;

• 1:牡丹江医学院基础医学院免疫学教研室

摘要(Abstract)：

目的探讨miR-9-3p对刀豆蛋白A(ConA)诱导所致小鼠慢性实验性肝损伤的影响。方法 ConA构建小鼠慢性实验性肝损伤模型,高通量测序筛查模型动物肝组织miRNA的差异表达。BALB/c小鼠40只,随机分成对照组、ConA损伤组、过表达组(ConA+慢病毒包装miR-9-3p组)、过表达对照组(ConA+慢病毒空载体组)每组10只;各模型组小鼠经尾静脉按8 mg/kg体重注射ConA每周1次(对照组注射等体积生理盐水),连续8周;过表达组于第7周经尾静脉注射,将慢病毒包装的miR-9-3p过表达质粒(2×10~7 TU/mL)注射到小鼠体内,每周1次,连续2周;过表达对照组注射等量慢病毒空载体;于ConA末次给药7 d后处死小鼠收集样本,电子天平检测肝脏指数变化,血清酶学检测AST,ALT水平,HE染色检测模型小鼠肝脏组织病理改变;蛋白印迹(Western Blot)检测模型小鼠肝脏组织中COL1A1及α-SMA蛋白的表达变化。结果高通量测序显示慢性肝损伤模型中miR-9-3p表达明显下调。在肝损伤鼠体内过表达miR-9-3p后,血清AST和ALT水平明显下降;HE染色检测观察到过表达的miR-9-3p明显减轻肝ConA诱导的肝脏组织病理损伤,与ConA损伤组比较,细胞肿胀坏死减弱,小叶结构恢复;Western Blot检测过表达miR-9-3p后COL1A1及α-SMA蛋白的表达明显减低。结论 miR-9-3p可减弱ConA诱导所致小鼠慢性实验性肝损伤。

关键词(KeyWords)： miR-9-3p;高通量测序;免疫性肝损伤;ConA

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省省属高等学校基本科研业务费支持项目(2020-KYYWF-0781;2018-KYYWFMY-0024)

作者(Author): 王闯;王猛;聂影;文怡欣;刘野;鄂志野;鞠宝玲;张红军;

Email:

DOI: 10.13799/j.cnki.mdjyxyxb.2021.02.001

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