银杏叶提取物对大鼠肝微粒体细胞色素P4502D6活性的影响THE EFFECT OF GINKGO BILOBA EXTRACT ON CYP2D6 ACTIVITY IN TAR LIVER
吴红;念红;吴琦;吴宜艳;刘桂莲;李伟阳;董凯;
摘要(Abstract)：
目的:观察银杏叶提取物(GBE)对大鼠肝细胞色素P450酶系统中CYP2D6活性的影响。方法:HPLC法测定大鼠尿液和肝微粒体中探针药物右美沙芬的含量。对照组和实验组大鼠分别经口给予生理盐水和银杏叶提取物两周,差速离心分离肝微粒体,HPLC法测定大鼠尿液和肝微粒体中CYP2D6探针药物的代谢率。观察银杏叶提取物对CYP2D6活性的影响。结果:实验组大鼠给予银杏叶提取物(100mg/kg),其尿样中右美沙芬的代谢率与对照组相比没有明显差别(P>0.05);实验组大鼠肝微粒体中加入右美沙芬(0.324mmol/L),其右美沙芬的代谢率与对照组相比没有明显差别(P>0.05);银杏叶提取物没有明显降低右美沙芬的代谢率(P>0.05)。结论:银杏叶提取物对CYP2D6酶无明显影响。
关键词(KeyWords)： 银杏叶提取物;肝微粒体;CYP2D6
基金项目(Foundation): 黑龙江省自然基金科研项目D2007104;; 黑龙江省卫生厅科研项目2006393
作者(Author): 吴红;念红;吴琦;吴宜艳;刘桂莲;李伟阳;董凯;
Email:
DOI: 10.13799/j.cnki.mdjyxyxb.2010.01.007
参考文献(References)：
- 1王金亭.银杏叶提取物的药用及食用价值[J].食品与药品,2006,8(11):73-74.
- 2Chan PC,Xia Q,Fu PP.Ginkgo biloba leave extract:biological,medicinal,and toxicological effects[J].J Environ Sci Health C En-viron Carcinog Ecotoxicol Rev,2007,25(3):211-244.
- 3Ahlemeyer B,Krieglstein J.Neuroprotective effects of Ginkgo biloba extract[J].Cell Mol Li fe Sci,2003,60(9):1779-1792.
- 4Yoshikawa T,Naito Y,K ondo M.Ginkgo biloba leaf extract:re-viewof biological actions and clinical applications[J].Antioxid Red-ox Signal,1999,1(4):469-480.
- 5Zhou W,Chai H,Lin PH,et al.Clinical use and molecular mech-anisms of action of extract of Ginkgo biloba leaves in cardiovascular diseases[J].Cardiovasc Drug Rev,2004,22(4):309-319.
- 6Bradford,M.M.Arapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal.Biochem1,1976;72:248.
- 7Moon J Y,Lee D W,Park K H,et al.Inhibition of7-ethoxycou-marin O-deethylase Activity in Rat Liver Microsomes by Naturally Occurring Flavomoids:Structure-activity Relationships.Xenobioti-ca,1998,28(2):117.
- 8秦达念,余白蓉,余运初.银杏叶提取黄酮对实验动物及人绒毛组织生殖功能的影响[J].中药新药与临床药理,2000,11(6):349.