李虹葳;许广义;李爽;宋高臣;李丽;

• 1:牡丹江医学院
• 2:牡丹江医学院机能学教研室

摘要(Abstract)：

目的:观察淫羊藿苷(icariin,ICA)后处理对心肌缺血/再灌注损伤的保护作用及其可能机制。方法:将24只家兔分为3组:假手术组、I/R组以及ICA后处理组。采用家兔在体模型,家兔麻醉后,开胸短暂结扎冠状动脉左前降支模拟缺血,缺血30min后松开结扎线进行再灌注,再灌注前10min耳缘静脉注射给药。灌注过程中检测左心室内压等心功能指标,再灌注180min后采血检测血清中SOD、MDA;随后处死动物,心脏染色测定心肌梗死面积,检测心肌组织中Bcl-2、Bax蛋白水平。结果:与假手术组比较,I/R组中LVSP、±dp/dtmax下降,LVEDP升高,HR降低;血清中SOD活性降低,MDA含量增高;Bcl-2、Bax蛋白表达量增高(P<0.01,P<0.05)。与I/R组比较,ICA后处理组中LVSP、±dp/dtmax升高,LVEDP下降;心梗面积减少;血清中SOD活性增高,MDA含量降低;Bcl-2蛋白表达量增高,Bax蛋白表达量降低,(P<0.05)。结论:ICA后处理对MIRI造成的心脏损伤具有保护作用,能明显改善心功能各项指标,缩小心梗面积,其保护机制与减轻心肌氧化应激反应、抑制心肌细胞凋亡有关。

关键词(KeyWords)：

Abstract:

Keywords:

基金项目(Foundation): 牡丹江医学院学生科研项目(编号:201205)

作者(Authors): 李虹葳;许广义;李爽;宋高臣;李丽;

DOI: 10.13799/j.cnki.mdjyxyxb.2014.06.006

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