乳腺癌患者血清中VEGF-C的表达及siRNA靶向抑制MCF-7细胞侵袭与转移的研究
孙平;刘艳翠;徐永良;
摘要(Abstract)：
目的:探讨VEGF-C(vascular endothelial growth factor-C)在乳腺癌患者血清中的表达及与肿瘤转移的关系;VEGF-CsiRNA对MCF-7中VEGF-C表达及细胞侵袭与转移的影响。方法:ELISA法检测30例乳腺癌及10名健康者空腹血清VEGF-C浓度;合成VEGF-CsiRN并采用Lipofectamine TM2000转染MCF-7细胞;RT-PCR法和免疫细胞化学检测VEGF-CmRNA和蛋白的表达;Transwell检测细胞侵袭情况。结果:乳腺癌患者血清中VEGF-C浓度明显升高,且发生淋巴结转移的VEGF-C浓度明显高于对照组(P<0.05);转染VEGF-CsiRNA的细胞VEGF-CmRNA和蛋白表达明显降低(P<0.05),侵袭能力也明显下降(P<0.05)。结论:VEGF-CsiRNA不仅能特异性降低VEGF-CmRNA及蛋白的表达,且能抑制MCF-7的侵袭和转移。
关键词(KeyWords)： VEGF-C;siRNA;乳腺癌;侵袭;转移
基金项目(Foundation): 黑龙江省自然科学基金项目(H201377);; 黑龙江省卫生厅科研项目(2012-285)
作者(Authors): 孙平;刘艳翠;徐永良;
DOI: 10.13799/j.cnki.mdjyxyxb.2014.06.022
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