李作武;郭继芳;游陆;

• 1:牡丹江市中医医院
• 2:牡丹江医学院附属红旗医院
• 3:武汉大学第二临床学院

摘要(Abstract)：

目的:观察肢体缺血后处理对兔心肌缺血再关注损伤后纤溶因子的影响。方法:健康新西兰大白兔随机分为3组,(l)S组,即假手术组,开胸后穿线套环,不收紧结扎线。(2)I/R组,结扎冠状动脉左前降支(LAD)30min,再灌注180min。(3)缺血后处理组(I-PostC组),结扎LAD 24 min时,用血管夹夹闭双侧股动脉5 min,松开1 min,再灌注直至180min。各组分别于结扎前、结扎30min、开放1h、开放3h取动脉血,5000转/min离心10min,取血清以酶联免疫吸附试验测定t-PA、PAI-1。结果:各组血浆中t-PA活性在缺血再灌期间呈进行性下降趋势,而PAI-1活性呈进行性升高趋势,再灌后,与I/R组t-PA(200.11±11.89pg/ml)和PAI-1(22.03±1.74ng/ml)比较,I-PostC组能显著对抗t-PA活性的降低(375.63±26.87pg/ml,P<0.01)和PAI-1活性的升高(18.12±1.20ng/ml)。结论:肢体缺血后处理能够改善纤溶/抗纤溶系统功能,抑制血栓的形成,从而保护缺血再灌损伤的心肌。

关键词(KeyWords)： 缺血后处理;缺血再灌注;纤溶因子

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李作武;郭继芳;游陆;

Email:

DOI: 10.13799/j.cnki.mdjyxyxb.2010.04.017

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