李作武;郭继芳;游陆;

• 1:牡丹江市中医医院
• 2:牡丹江医学院附属红旗医院
• 3:武汉大学第二临床学院

摘要(Abstract)：

目的:观察肢体缺血后处理对兔心肌缺血再关注损伤后纤溶因子的影响。方法:健康新西兰大白兔随机分为3组,(l)S组,即假手术组,开胸后穿线套环,不收紧结扎线。(2)I/R组,结扎冠状动脉左前降支(LAD)30min,再灌注180min。(3)缺血后处理组(I-PostC组),结扎LAD 24 min时,用血管夹夹闭双侧股动脉5 min,松开1 min,再灌注直至180min。各组分别于结扎前、结扎30min、开放1h、开放3h取动脉血,5000转/min离心10min,取血清以酶联免疫吸附试验测定t-PA、PAI-1。结果:各组血浆中t-PA活性在缺血再灌期间呈进行性下降趋势,而PAI-1活性呈进行性升高趋势,再灌后,与I/R组t-PA(200.11±11.89pg/ml)和PAI-1(22.03±1.74ng/ml)比较,I-PostC组能显著对抗t-PA活性的降低(375.63±26.87pg/ml,P<0.01)和PAI-1活性的升高(18.12±1.20ng/ml)。结论:肢体缺血后处理能够改善纤溶/抗纤溶系统功能,抑制血栓的形成,从而保护缺血再灌损伤的心肌。

关键词(KeyWords)： 缺血后处理;缺血再灌注;纤溶因子

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 李作武;郭继芳;游陆;

DOI: 10.13799/j.cnki.mdjyxyxb.2010.04.017

参考文献(References)：

• [1]ParssonH,HolmbergA,SiegbahnA,et al.Activation of coagula-tio-nandfibrinolyticsystemsin patients with CLI is not normalizedaftersurgical revascularization.Eur J VascEndovasc Surg,2004,27(2):186-92.
• [2]SehumaeherHC,MeyersPM,YavagalDR,etal.Endovascularmechan-ical thrombectomyofanoccludedsuperiordivisionbranchoftheleft MCAforacutecardioembolicstroke.CardiovascInterventRadio,2003,26(3):305-8.
• [3]RoelofsJJ,RousehopKM,LeemansJC,et al.Tissue-type plasmino-gen activatormodulatesinflammatoryresponsesandrenal function in is-chemia reperfusion injury.JAmSovNephrol.2006,17(1):131-40.
• [4]NagaiN,SuzukiY,VanHoefB,et al.Effectsof plasminogenactivatorinhibitor-1onischemic braininjuryin permanentandthromboticmiddle cerebralarteryocclusionmodelsinmice.JThrombHaemost,2005,3(7):1379-84.
• [5]GorlachA.Redoxregulationofthe coagulation cascade.Antioxid Re-doxSignal.2005,7(9-10):1398-404.
• [6]GorlachA,Berehner-PfannschmidtU,WotzlawC,et al.Reactiveox-ygen speciesmodulate HIF-1 mediatedPAI-1expression:involve-mentofthe GTPaseRacl.ThrombHaemost,2003,89(5):926-35.
• [7]CiterioG,GalliD,CadoreB,etal.How to improve ischemic stroketreatment in the fibrinolysisera..MinervaAnestesiol,2006,72(6):07-12.
• [8]HalkinA,RothA,JonasM,et al..Sulfonylureasarenot associatedWithinereasedmortalityindiabetietreatedwiththrombolysis for acutemyocardial infarction.ThrombThrombolysis.2001,12(2):177-84.
• [9]Stief TW.The physiologyand pharmacologyofsingletoxygen.Med Hy-potheses.2003,60(4):567-72.