构建人ICAM-1真核表达载体的实验研究CONSTRUCTION AND EXPRESSION OF HUMAN INTERCELLULAR ADHESION MOLECULE-1 EXPRESSION VECTOR
陈志鸿;静雅杰;宋宝辉;
摘要(Abstract)：
目的:构建人ICAM-1-1真核表达载体pcDNA3.1(-)-ICAM-1。方法:设计特异性引物,利用PCR技术扩增出ICAM-1全编码区基因片段,插入到真核表达载体pcDNA3.1(-)中。结果:PCR扩增得到人成熟ICAM-1的cDNA片段大小为1800bp,重组子利用限制性内切酶进行酶切鉴定和DNA序列分析得到真核表达载体pCDA3.1(-)-ICAM-1。结论:成功构建了人ICAM-1真核表达载体,为进一步建立稳定转染ICAM-1的细胞株以及研究ICAM-1及其受体的生物学功能提供了条件。
关键词(KeyWords)： 人ICAM-1-1;限制性内切酶;真核表达载体
基金项目(Foundation): 牡丹江医学院优秀青年基金[2002-28]
作者(Author): 陈志鸿;静雅杰;宋宝辉;
Email:
DOI: 10.13799/j.cnki.mdjyxyxb.2007.04.009
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