陈志鸿;静雅杰;宋宝辉;

• 1:牡丹江医学院生物教研室
• 2:牡丹江师范学院基础教育分院
• 3:牡丹江医学院生物教研室 黑龙江牡丹江157011
• 4:黑龙江牡丹江157011

摘要(Abstract)：

目的:构建人ICAM-1-1真核表达载体pcDNA3.1(-)-ICAM-1。方法:设计特异性引物,利用PCR技术扩增出ICAM-1全编码区基因片段,插入到真核表达载体pcDNA3.1(-)中。结果:PCR扩增得到人成熟ICAM-1的cDNA片段大小为1800bp,重组子利用限制性内切酶进行酶切鉴定和DNA序列分析得到真核表达载体pCDA3.1(-)-ICAM-1。结论:成功构建了人ICAM-1真核表达载体,为进一步建立稳定转染ICAM-1的细胞株以及研究ICAM-1及其受体的生物学功能提供了条件。

关键词(KeyWords)： 人ICAM-1-1;限制性内切酶;真核表达载体

Abstract:

Keywords:

基金项目(Foundation): 牡丹江医学院优秀青年基金[2002-28]

作者(Authors): 陈志鸿;静雅杰;宋宝辉;

DOI: 10.13799/j.cnki.mdjyxyxb.2007.04.009

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