杨月;张炜婷;刘金丽;王红艳;刘野;郭素芬;

• 1:牡丹江医学院病理教研室
• 2:牡丹江医学院附属红旗医院感染科
• 3:牡丹江市第二人民医院病理科

摘要(Abstract)：

目的从细胞水平上探讨顺铂(DDP)对乳腺癌MDA-MB-231细胞系增殖的影响,以及其可能机制。方法使用不同浓度(0、1.25、2.5、5、10、20、40μmol/L)的顺铂作用于乳腺癌细胞系MDA-MB-231;CCK-8方法检测各浓度组细胞24h后的增殖情况;选取对细胞增殖影响显著的顺铂浓度(0、10、20、40μmol/L)作用MDA-MB-231细胞24h,收集细胞并采用Western blot方法检测CXCR4的表达;采用Notch1抑制剂MK0752预处理MDA-MB-231细胞2h后,再用浓度为20μmol/L的顺铂作用于预处理的MDA-MB-231细胞24h后收集细胞,并采用Western blot方法检测阻断效果以及CXCR4的表达。结果顺铂可以抑制乳腺癌细胞的增殖(P<0.05);与对照组相比,随着浓度给药浓度的增加,CXCR4的表达显著降低(P<0.05);阻断Notch1(阻断组)后,与未阻断组(Notch1未被阻断)相比,CXCR4的表达显著降低(P<0.001)。结论化疗药物顺铂部分通过Notch1抑制CXCR4的表达,从而抑制乳腺癌细胞增殖。

关键词(KeyWords)： 顺铂;Notch1;CXCR4;乳腺癌

Abstract:

Keywords:

基金项目(Foundation): 牡丹江医学院研究生创新基金(2018YJSCX-23MY);; 牡丹江市科学技术计划项目(Z2018s036);; 黑龙江省自然基金(LH2019H119);; 黑龙江省高校基本科研业务费(2018-KYYWFMY-0006)

作者(Authors): 杨月;张炜婷;刘金丽;王红艳;刘野;郭素芬;

DOI: 10.13799/j.cnki.mdjyxyxb.2020.02.013

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